MxP® Quant 500 kit validated on Sciex Triple Quad 7500 LC MS/MS System – bringing targeted metabolomics to the next generation of SCIEX triple quadrupoles
Metabolomics is an invaluable tool for investigating and understanding the complex mechanisms involved in cellular biology, opening the door to new insights in health, nutrition and disease.
Now, scientists and researchers can use metabolomics techniques with greater ease and efficiency, thanks to the MxP® Quant 500 metabolite profiling kit being validated for use with the new SCIEX 7500 system.
The MxP® Quant 500 is the leading broad metabolite profiling kit for use with liquid chromatography-tandem mass spectrometry (LC/MS). As the most comprehensive metabolomics kit on the market, it offers a ready-to-use solution with a simple automated workflow for the quality-controlled analysis of 630 small molecules and lipids from 26 compound classes.
With more than 230 predefined, biologically relevant metabolite sums and ratios, it facilitates advanced biological data interpretation with increased statistical power.
Validating the MxP® Quant 500 kit with SCIEX 7500
The SCIEX 7500 system is the latest triple quadrupole from SCIEX. In addition to new SCIEX OS software, the ion source and other hardware components have been redesigned. The MxP® Quant 500 kit was validated on earlier SCIEX systems and widely used since its launch in 2019.
After extensive testing and method parameter optimization, we can now confirm that the new 7500 system, with its improved sensitivity and precision, is an ideal match for the Quant 500 kit.
For the analysis, 13 polar small molecule classes were analyzed by ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS), while hexoses and 12 lipid classes were analyzed by flow injection analysis-tandem mass spectrometry (FIA-MS/MS).
The Quant 500 kit was validated on a UHPLC coupled to a SCIEX 7500 platform using the high flow rate ion source. The validation was carried out across three kit plates run on three separate days, consisting of three concentration levels of plasma, fecal, and urine quality control (QC) samples.
Pooled samples of each matrix type representing typical unknown samples were also run on each plate. The scope of the validation provided detailed information regarding intra and inter day kit performance, accuracy, reproducibility and stability. The pooled matrix samples provided the expectations for metabolite coverage in real study samples.
The results were impressive, demonstrating improved compatibility between the Quant 500 kit and the new SCIEX platform.
Summary of exceptional performance:
- Using the biocrates standard level QC sample as a reference, excellent precision was observed across all three plates with <6% average CV within each plate and <15% average CV across all plates for analytes detected above the limit of detection (LOD).
- Analyte coverage detectable above LOD compared favorably with other instruments. In a pooled human plasma sample, 499 metabolites were detected above LOD. n a pooled human fecal sample, 447 metabolites were detected above LOD. In pooled urine, 66 metabolites were detected above LOD, owing to the low lipid abundance in this matrix.
- Calibration standards showed good accuracy across the full range of concentrations for each analyte with quadratic curves fittings of r2=0.99.
As with its predecessors, the kit performs exceptionally well on the latest SCIEX instrument, offering an effective platform for those looking to upgrade and those just getting started into quantitative metabolomics.