AbsoluteIDQ® Bile Acids kit

The benchmark solution to assess host-microbiota interaction

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease.

The AbsoluteIDQ Bile Acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

Bile-Acids

AbsoluteIDQ® Bile acids kit

The benchmark solution to assess host-microbiota interaction

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease.

The AbsoluteIDQ bile acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease.

The Biocrates® Bile Acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

Ready-to-use_Kitbox

All in one box

  • Includes all necessary reagents and methods
Functional-Microbiomics_Mikroben

High throughput

  • Up to 400 samples per instrument per week
Ready-to-use_Kitbox

Proven reproducibility

Superior-Data-Quality_Zielscheibe

Superior data quality

  • Outstanding accuracy and precision of results
AbsoluteIDQ-Bile-Acids-kit

Kit components

Reagents and consumables
  • Patented 96-well filter plates with pre-set internal standards
  • Calibration standards and quality controls
  • Test samples for system suitability test
Methods and protocols
  • Quick-start guide and user manual
  • Sample preparation protocol
  • Instrument-specific methods

Kit requirements

WebIDQ™ – Workflow manager
  • Cloud-based software (on-premise option also available)
  • Complete experimental process guidance
  • Automated quantification, technical validation and report generation
  • MetaboINDICATOR™ with biologically relevant metabolite sums and ratios
LC column and MS systems
  • Compatible with Agilent 6495 C; SCIEX 6500+, 6500, 5500+, 5500, 4500, 4000; Waters TQ-S, TQ-S micro; Thermo TSQ Vantage
  • Validated LC column
  • Compatible with ultra high performance liquid chromatography (UHPLC)
Covered bile acids
  • 10 primary bile acids: produced by the liver (CA, GCA, TCA, CDCA, GCDCA, TCDCA, α-MCA, β-MCA, ω-MCA, TMCA(α+β))
  • 10 secondary bile acids: produced by gut bacteria from primary bile acids (DCA, GDCA, TDCA, LCA, GLCA, TLCA, UDCA, GUDCA, TUDCA, HDCA)

Is this the right kit for my instruments and applications?

For research use only | not for use in diagnostic procedures.

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