Metabolomic Phenotyping Services

Expert support from study design to actionable results

Our Metabolomic Phenotyping Services enable a quality-controlled, highly reproducible, quantitative analysis across the metabolite spectrum. Our expert services offer full support in every step of your project, including sample preparation, shipment, project management, statistical analysis, and biomedical data interpretation.

Key benefits

Comprehensive analytical portfolio

  • Best fit for every research question
  • Experience with wide range of matrices
  • Customized assay development

Short turn-around times

  • High-quality results typically < 12 weeks after sample receipt
  • Expert analysis in specialized routine laboratory
  • Experience with (pre-analytical) sample handling

Project management

  • Support from study design to data interpretation
  • Experience with discovery and validation studies
  • Guidance in state-of-the-art sampling, storage, and shipment

Biostatistics & data interpretation

  • Established institutional knowledge
  • Profound expertise in biostatistics
  • Biomedical interpretation & metabolic pathway visualization

Targeted & quantitative metabolomics at your service

Comprehensive analytical portfolio

Our Metabolomic Phenotyping Services allow for customized selection of assays which cover a wide range of physiological processes

ApproachAssay NameNumber & Class of Metabolites
Kit-based assaysMxP® Quant 500 kitUp to 107 small molecules & 523 lipids
AbsoluteIDQ® p400 HR kitUp to 43 small molecules & 365 lipids
AbsoluteIDQ® p180 kitUp to 43 small molecules & 145 lipids
AbsoluteIDQ® Stero17 kitUp to 17 steroid hormones
Biocrates® Bile Acids kitUp to 20 bile acids
Specialized
targeted assays
Eicosanoid assayUp to 17 eicosanoids
Fatty acid assay (total / free)Up to 31 / 32 fatty acids
Lipid assayUp to 326 lipids
Oxysterol assayUp to 18 free (oxy-) sterols
Energy metabolism assayUp to 21 energy metabolism intermediates
NEW Tryptophan metabolism assayUp to 17 metabolites from the tryptophan pathway

For more information on the NEW Tryptophan metabolism assay, please click here.

Altogether, our assays cover more than 1,000 metabolites from over 30 classes listed below.

 

Metabolite portfolio

Small molecules

  • Alkaloids
  • Amine oxides
  • Amino acids
  • Amino acid related
  • Bile acids
  • Biogenic amines
  • Carbohydrates and related
  • Carboxylic acids
  • Cresols
  • Eicosanoids
  • Energy metabolism intermediates
  • Fatty acids
  • Hormones and related
  • Indoles and derivatives
  • Nucleobases and related
  • Vitamins and cofactors

Lipids

  • (Acyl-)Carnitines
  • Phosphatidylcholines
  • Lysophosphatidylcholines
  • Phosphatidylethanolamines
  • Lysophosphatidylethanolamines
  • Phosphatidylglycerols
  • Lysophosphatidylglycerols
  • Phosphatidylserines
  • Sphingomyelins
  • Ceramides
  • Dihydroceramides
  • Hexosylceramides
  • Dihexosylceramides
  • Trihexosylceramides
  • Cholesteryl esters
  • Diglycerides
  • Triglycerides
  • Free (oxy-)sterols

Applicability to species and biological matrices

Sample preparation methods can strongly influence the yield of extraction of metabolites and the overall sensitivity of metabolomic experiments. We are experienced in handling and preparing samples from a broad range of biological matrices from various species.

Below is a non-exhaustive list of the species / matrices processed over the years. To check on the current recommendations for your species or matrix of interest, please contact us.

SpeciesHuman, mouse, rat, chicken, pig, sheep, cow, dog, primate, guinea pig, hamster, fish, 
C. elegans, E. coli, P. pastoris
MatrixBody FluidsPlasma, serum, urine, whole blood, Interstitial fluid ("blister liquid") , saliva, cerebrospinal fluid (CSF), bronchoalveolar lavage fluid (BALF), milk
TissueLiver, kidney, brain, lung, prostate, ovary, breast, colon, pancreas, muscle, skin
Cell CultureCHO cells, cultivated cancer cells, inclusion bodies, media and supplements (soy and yeast hydrolysates), neuronal cells
OthersFeces, dried blood/plasma spots, cecal content

Biostatistics & data interpretation

Confident decision making based on metabolomics requires comprehensive statistical analysis and informed interpretation of the analytical data.
Biocrates generates for you a customized data report, delivering actionable results derived from robust and reproducible metabolomic data. With our profound scientific expertise in biostatistics and metabolomics we offer three reporting options:

* Currently only available for MxP® Quant 500 service.

Project management

In metabolomics, an elaborate study design is crucial to obtain meaningful, actionable results.
Therefore, our tailor-made support services include project management and guidance in:

  • Selection of scientific question & most suitable metabolomic assay(s)
  • Study design & feasibility
  • Sample handling: collection, processing, storage, shipment

We offer guidance on best practices to handle your samples for measurement with our Services. For specific recommendations on blood sample preparation for targeted metabolomics, see our Sample Preparation Guidelines. For recommendations on how to collect and handle tissue, cell culture samples and more, please contact us.

For Research Use Only. Not for use in diagnostic procedures.