AbsoluteIDQ® p400 HR kit

Best resolution

The AbsoluteIDQ® p180 kit is a simple and high-throughput tool for basic, clinical, and epidemiological research. The kit provides scientists with highly reproducible metabolomics data to confidently obtain detailed knowledge about the metabolic phenotypes in their studies. The successful application of the kit is demonstrated in hundreds of published articles making it the most frequently chosen metabolomics approach of its kind.

AbsoluteIDQ® p400 HR kit

Best resolution

The AbsoluteIDQ® p400 HR kit is the first ready-to-use, standardized solution for broad lipid and metabolic profiling on Q Exactive™ mass spectrometers. It provides quantification of more than 400 metabolites and lipids from 11 compound classes covering central metabolic pathways.



  • Implemented within one day
  • All in one box: no method or reagent development required
  • For a broadly established standard instrument base
p400 HR simple workflow

High throughput & minimal sample volume

  • Up to 240 samples per week and instrument
  • 10 µL needed for most biofluids
    (validated for human EDTA plasma)
p400 HR high throughput

Simple, guided, automated workflow

  • Workflow manager guided procedure for enhanced process safety
  • Automated signal identification, quantification, and technical validation

Superior data quality

  • Quantitative results
  • Proven analytical performance: excellent accuracy and precision
  • Reproducibility proven by international ring trial
AbsoluteIDQ-p400 HR-kit

Kit components

Reagents and consumables
  • Patented 96-well filter plates with pre-set internal standards
  • Calibration standards and quality controls
  • Test samples for system suitability test
Methods and protocols
  • Quick-start guide and user manual
  • Sample preparation protocol
  • Instrument-specific methods

Kit requirements

WebIDQ™ – Workflow manager
  • Cloud-based software (on-premise option also available)
  • Complete experimental process guidance
  • Automated quantification, technical validation and report generation
  • MetaboINDICATOR™ with biologically relevant metabolite sums and ratios
LC column and MS systems
  • Compatible with Thermo Exactive, Exactive focus, Exactive Plus, Exactive HF
  • Validated LC column
  • Compatible with ultra high performance liquid chromatography (UHPLC)

Metabolite coverage

Small molecules
  • Amino acid (21)
  • Biogenic amines (21)
  • Hexose (1)
Polar lipids
  • Phosphatidylcholines (172)
  • Lysophosphatidylcholines (24)
  • Sphingomyelins (31)
  • Ceramides (9)
Neutral lipids
  • Acylcarnitines (55)
  • Cholesteryl esters (14)
  • Diglycerides (18)
  • Triglycerides (42)

Is this the right kit for my instruments and applications?

For research use only | not for use in diagnostic procedures.

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