AbsoluteIDQ® p400 HR Kit

Best resolution

The AbsoluteIDQ® p400 HR kit is the first ready-to-use, standardized solution for broad lipid and metabolic profiling on high resolution, accurate mass (HRAM) Q Exactive™ mass spectrometers. It provides quantification of up to 408 metabolites and lipids from 11 compound classes covering central metabolic pathways.

Ready-to-use

  • Implemented within one day
  • All in one box: no method or reagent development required
  • For a broadly established standard instrument base

High throughput & minimal sample volume

  • Up to 240 samples per week and instrument
  • 10 µL needed for most biofluids
    (validated for human EDTA plasma)

Simple, guided, automated workflow

  • Workflow manager guided procedure for enhanced process safety
  • Automated signal identification, quantification, and technical validation

Superior data quality

  • Quantitative results
  • Proven analytical performance: excellent accuracy and precision
  • Reproducibility proven by ring trial (Thompson et al.)

Kit components

Reagents & consumables

  • Patented 96-well filter plate
  • Internal & calibration standards
  • Quality controls
  • Testmix

Methods & protocols

  • Sample preparation protocol
  • Instrument-specific acquisition & quantification methods
  • System suitability test

Workflow manager MetIDQ

  • Process guidance
  • Automated technical validation
  • Basic statistics (optional)

Metabolite coverage

Small molecules

  • Amino acid (21)
  • Biogenic amines (21)
  • Hexose (1)

Polar lipids

  • Phosphatidylcholines (172)
  • Lysophosphatidylcholines (24)
  • Sphingomyelins (31)
  • Ceramides (9)

Neutral lipids

  • Acylcarnitines (55)
  • Cholesteryl esters (14)
  • Diglycerides (18)
  • Triglycerides (42)