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The gold standard in targeted metabolomics

Metabolomics is a powerful tool to answer the multidimensional questions of biology. From just a few microliters of sample, the phenotype of an individual can be uncovered and explored. Metabolomics is key to generating high-quality and reliable data that is mandatory for today’s biological research and individualized health.

Technology brochure

The gold standard in quantitative metabolomics

Metabolomics is a powerful tool to answer the multidimensional questions of biology. From just a few microliters of sample, the phenotype of an individual can be uncovered and explored. Metabolomics is key to generating high-quality and reliable data that is mandatory for today’s biological research and individualized health.
The central theme of biocrates’ metabolomics profiling products is providing quantitative, reproducible assays designed in an easy and ready-to-use kit format. Quantitative, standardized and quality-controlled methods combined with a standardized format guarantee robust and reproducible results – the cornerstones of any scientific finding.

biocrates kits in your laboratory

We democratize metabolomics

– All-in-one metabolomics and lipidomics solution

– Ready to use and easy to measure

– Accessible advanced analysis

– Peace of mind with automated quality checks and guided expert support

Work smarter – not harder

– 1,400+ analytically validated biomarkers [µM] in a single assay

– Robust, reproducible, scalable, time and resource saving

– Global integration into laboratory network by cloud-based solutions

– Cost per analyte that is meeting all budgets

Get metabolomics done right

– Broad compatibility with data analysis tools

– FAIR compliant with complete ownership of data

– Data security and GDPR compliant

– Benchmark against QMDB reference database

Standardized workflow

biocrates’ kits include years of experience in targeted metabolomics. These ready-to-use kits deliver quality-controlled and reproducible quantification of metabolites in biological samples by following only five steps. WebIDQ, a user-friendly workflow manager software, guides the user through the whole process, from sample registration to biological interpretation of the results.

Sample preparation (wet work only here)

Perform system suitability test
Derivatize and extract your samples
Pipette samples, standards, and QCs onto the plate

Data collection

Run plates with LC- and FIA-MS
Instrument-specific methods are provided

Quantitation & Validation

Automated peak identification and quantification
Automated sensitivity and accuracy check
In-built data normalization suite

Statistics & Interpretation

Perform advanced statistics with MetIDQ™ StatPack OR
Export from MetIDQ™ to your statistics software of choice
For MxP® Quant 500: support your data interpretation with the innovative MetaboINDICATOR™ add-on to MetIDQ™.

Insight &Understanding

Make decisions based on standardized and reproducible metabolomic data
Compare your results with published kit data from other laboratories

Peace of mind

We’ve taken care of the technical details so that you can focus on the science. Spend more time discovering biological implications and planning for your next challenges to tackle.

Technology brochure

Metabolomics kit products

MxP^®Quant 500 XL

The largest combination of lipids and small molecules for quantitative metabolic profiling in a single kit

MxP^® Quant 500

The comprehensive kit to study mechanisms of disease, nutrition, and the microbiome

AbsoluteIDQ^® p400 HR

The high performance, high resolution tool for targeted metabolomics profiling

AbsoluteIDQ^® p180

The gold standard in quantitative metabolomics for all fields of research

AbsoluteIDQ^® Stero17

The weightlifter for targeted steroid hormone screening

AbsoluteIDQ^®Bile acids

The benchmark solution to assess host-microbiota interaction

What is in a kit?

The plate

biocrates’ kits are designed for high throughput experiments in a standard 96 well plate format allowing up to 82 user samples per plate. To accommodate users with smaller sample sizes, half sized and training plates are also available. The plates incorporate a novel insert design allowing all preparation and cleanup to be performed directly on the plate. A top filter plate is incorporated to remove proteins that precipitate during preparation. Our proprietary design allows for more efficient drying and extraction improving the quality of results. At the end of preparation, samples are collected in the bottom 96-well plate which can be run directly in your LC-MS autosampler.

Components

biocrates kits are equipped with all of the required components for a robust, quantitative analysis. For quantification, seven levels of calibration standards and isotope-labeled internal standards are used. For performance validation and data normalization, plasma-based quality control samples at three concentration levels are provided and directly incorporated with our software. All reagents come as lyophilized solids in color-coded vials for quick resuspension and easy preparation.

Methods

Each of our kits has been developed and validated for specific LC-MS systems from a number of vendors. We carefully tailor and optimize each method to provide the best possible performance for every instrument. The kits include all vendor-specific acquisition methods. This includes MRM transitions, ion source parameters and chromatography settings to make setup quick and easy without the need for extensive method development. After data collection, processing methods are also provided to make data processing quick and robust. Data processing and validation are directly incorporated with biocrates’ MetIDQ software.

Matrices and sample preparation

While specifically validated for plasma and serum samples, our kits can be used for any samples containing endogenous metabolites. We have developed substantial experience over the years in sample preparation and matrix use.

We offer guidelines on the preparation of blood samples as well as application notes on the preparation of fecal or urine samples and many more. Contact our support in case you have questions on sample preparation or non-standard matrices.

WebIDQ software

WebIDQ is the next evolution of biocrates’ metabolomics workflow management and data processing software. With an intuitive user interface, WebIDQ guides users from sample registration through quantification and reporting.

Building on the proven functionality of MetIDQ software, WebIDQ is designed specifically for a cloud environment making data analysis efficient from every device. New features such as automated peak picking and MetaboINDICATOR extensions make this software the go-to companion for quantitative metabolomics.

A WebIDQ subscription is needed to run biocrates kits and to process kit-generated data.

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“Our Metabolomics Core is involved in many long-term projects. Such projects require the generation of reliable and comparable data over a long period of time, so data quality is of paramount importance to us. We rely on the biocrates platform’s high-quality workflow and data analysis pipeline, which helped us eliminate variability and generate reproducible data. Their technology has also proven to be highly versatile and we could generate amazing insights in different indications using a wide spectrum of sample matrices (e.g. biofluids, tissues, cells). Therefore, I highly recommend the biocrates platform to any researcher looking to improve their data quality or just getting started in metabolomics.”

Dr. Gernot Poschet

Executive Director, Metabolomics Core Technology Platform, Heidelberg University & German Cancer Research Center