Oxysterol assay
Tryptophan metabolism assay
Tryptophan metabolism plays a critical role that encompasses the modulation of immune responses, the regulation of growth and the impact on mood as well as the behavior. This makes it a crucial candidate for both identifying disease biomarkers and developing therapeutic strategies.
The challenge
Oxysterols occur at very low concentrations and can easily form autooxidation artefacts, which makes accurate measurement difficult. Their structural similarity and need for precise isomer resolution add to the complexity, especially in complex biological samples.
The solution
Our experts have developed an accurate and robust assay that reliably quantifies low abundance oxysterols, minimizes autooxidation artefacts through optimized sample handling and quality control, and achieves confident isomer specific separation using a highly sensitive LC MS/MS workflow.
Quantifying key regulators of cholesterol metabolism
Oxysterols are powerful signaling molecules that sensitively reflect cholesterol metabolism, inflammation, oxidative stress, and neurodegeneration. Even minimal shifts in their concentrations can reveal highly specific biomarker patterns across a wide range of disease areas.
Designed for both biomedical research and pharmaceutical development, this assay delivers the accuracy, robustness, and biological insight required to uncover mechanisms, evaluate drug effects, and identify new biomarker signatures with confidence.
Comprehensive coverage
- Quantifies a broad panel of free oxysterols and cholesterol derived intermediates
- Captures critical steps across cholesterol metabolism pathways
Versatile applications
- Delivers precise oxysterol profiles suitable for pharmacodynamic and MoA analyses
- Ideal for studies in lipid metabolism, inflammation, and neurodegeneration
Reliable data quality
- Optimized LC MS workflow delivers consistent, high quality data
- 7-point calibration with internal standards ensures accurate quantification
Expert scientific support
- Expert support throughout study design, sample handling, and statistical data analysis
- Insightful interpretation linking oxysterol patterns to biological pathways
Covered free (oxy-) sterols
- Cholesterol
- 7-Dehydrocholesterol
- Desmosterol
- 24,25-Dihydrolanosterol
- 5α,6β-Dihydroxycholestanol (THC)
- 5α,6α-Epoxycholesterol
- 5β,6β-Epoxycholesterol
- 24,25-Epoxycholesterol
- 7α-Hydroxycholestenone
- 4β-Hydroxycholesterol
- 7α -Hydroxycholesterol
- 7β-Hydroxycholesterol
- 22R-Hydroxycholesterol
- 24S-Hydroxycholesterol
- 25-Hydroxycholesterol
- 27-Hydroxycholesterol
- 7-Ketocholesterol
- Lanosterol
