AbsoluteIDQ^® Bile Acids kit

AbsoluteIDQ^® Bile acids kit

The benchmark solution to assess host-microbiota interaction

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease.

The AbsoluteIDQ bile acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease. The Biocrates® Bile Acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

The benchmark solution to assess host-microbiota interaction

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease.

The AbsoluteIDQ Bile Acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

All in one box

Includes all necessary reagents and methods

High throughput

Up to 400 samples per instrument per week

Proven reproducibility

Excellent inter-laboratory comparability proven in international ring trial

Superior data quality

Outstanding accuracy and precision of results

Kit components

Standards and consumables

Patented 96-well filter plates with pre-set internal standards
Calibration standards and quality controls
Test samples for system suitability test

Methods and protocols

Quick-start guide and user manual
Sample preparation protocol
Instrument-specific methods

Kit requirements

WebIDQ™ – Workflow manager

Cloud-based software (on-premise option also available)
Complete experimental process guidance
Automated quantification, technical validation and report generation
MetaboINDICATOR™ with biologically relevant metabolite sums and ratios

LC column and MS systems

Compatible with Agilent 6495 C; SCIEX 6500+, 6500, 5500+, 5500, 4500, 4000; Waters TQ-S, TQ-S micro; Thermo TSQ Vantage
Validated LC column
Compatible with ultra high performance liquid chromatography (UHPLC)

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MetaboINDICATOR™

Translate metabolomics into knowledge with one click

Calculating and understanding metabolite sums and ratios makes your biological data interpretation easier and more comprehensive. That is why we created MetaboINDICATOR™ as an add-on tool to WebIDQ. With one click you can find out how metabolite sums and ratios are associated with related biological functions so your data has meaningful impact.

MetaboINDICATOR™ is more than an additional dimension to your data analysis; it unravels the scientiﬁc context behind your data. All in one click.

More about MetaboINDICATOR