AbsoluteIDQ® p400 HR kit

Best resolution

The AbsoluteIDQ® p400 HR kit is the first ready-to-use, standardized solution for broad lipid and metabolic profiling on Q Exactive™ mass spectrometers. It provides quantification of more than 400 metabolites and lipids from 11 compound classes covering central metabolic pathways.

AbsoluteIDQ p400 HR kit

MxP® Quant 500 kit validated on Sciex Triple Quad 7500 LC MS/MS System

Best resolution

The AbsoluteIDQ p400 HR kit is the first ready-to-use, standardized solution for broad lipid and metabolic profiling on Q Exactive™ mass spectrometers. It provides quantification of more than 400 metabolites and lipids from 11 compound classes covering central metabolic pathways.

Ready-to-use

Implemented within one day
All in one box: no method or reagent development required
For a broadly established standard instrument base

High throughput & minimal sample volume

Up to 240 samples per week and instrument
10 µL needed for most biofluids
(validated for human EDTA plasma)

Simple, guided, automated workflow

Workflow manager guided procedure for enhanced process safety
Automated signal identification, quantification, and technical validation

Superior data quality

Quantitative results
Proven analytical performance: excellent accuracy and precision
Reproducibility proven by international ring trial

Standards and consumables

Patented 96-well filter plates with integrated internal standards
Calibration standards and quality controls
Test samples for system suitability test

Methods and protocols

Quick-start guide and user manual
Protocols for different matrices
Instrument-specific methods

WebIDQ – Workflow manager

Cloud-based software (on-premise option also available)
Complete experimental process guidance
Automated quantification, technical validation and report generation
MetaboINDICATOR™ with biologically relevant metabolite sums and ratios

LC column and MS systems

Compatible with Thermo Exactive, Exactive focus, Exactive Plus, Exactive HF
Validated LC column
Compatible with ultra high performance liquid chromatography (UHPLC)

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Small molecules

Amino acid (20)
Biogenic amines (21)
Hexose (1)

Polar lipids

Phosphatidylcholines (172)
Lysophosphatidylcholines (24)
Sphingomyelins (31)
Ceramides (9)

Neutral lipids

Acylcarnitines (11)
Cholesteryl esters (14)
Diglycerides (18)
Triglycerides (42)

Download complete list

Literature references

Cerebrospinal fluid metabolic profiling in oncology

Jang S, Gwak H-S, Lee K-Y, Lee JH, Kim K-H, Kim JH et al. Exploratory profiling of metabolites in cerebrospinal fluid using a commercially available targeted LC-MS based metabolomics kit to discriminate leptomeningeal metastasis. Cancer & metabolism 2025; 13(1):2.
DOI: 10.1186/s40170-024-00367-x.

MetaboINDICATOR predicts therapy response in cohort studies (BACHB, FUEL)

Cedars A, Manlhiot C, Chinni BK, Opotowsky AR, Becker K, Le A et al. Methylated Arginine Metabolites as Biomarkers for Clinical Status and Response to Type 5 Phosphodiesterase Inhibition in Patients With Fontan Circulation. Journal of the American Heart Association 2025; 14(7):e038061.
DOI: 10.1161/JAHA.124.038061.

Metabolomics of human lymph samples

Mah MS, Cao E, Anderson D, Escott A, Tegegne S, Gracia G et al. High-fat feeding drives the intestinal production a nd assembly of C16:0 ceramides in chylomicrons. Science advances 2024; 10(34):eadp2254.
DOI: 10.1126/sciadv.adp2254.

taboINDICATOR

Translate metabolomics into knowledge with one click

Calculating and understanding metabolite sums and ratios makes your biological data interpretation easier and more comprehensive. That is why we created MetaboINDICATOR as an add-on tool to WebIDQ. With one click you can find out how metabolite sums and ratios are associated with related biological functions so your data has meaningful impact.

MetaboINDICATOR is more than an additional dimension to your data analysis; it unravels the scientiﬁc context behind your data. All in one click.

More about MetaboINDICATOR